been away

Well I've been ignoring this blog abit but I;ve been busy figuring in why the Sult in the zebra danio's is giving me a negitive image on my western blot. While a 20lbs cat seems to find that its okay to sleep on the keyboard while I type. Stupid cant uh I'll be alone in the lab on monday and will be alone until after the half way past the week.

A long day

well i did the plate reading assay quickly and got the right results unlike the last run which didn't even come out as linear. Th only down note is I kept screwing up the TBS buffer for monday its really sad on my part oh well I'll relax over the week end a look over mondays work. I hope someone feed the fish as the school was closed by the time I got to the main campus. Also the ATC building is a daycare center and I'm still paying for it hell charge the parents if they bring the kids... no that mean

Triumphant return to T107

Finally I'm back in my favorite room. Today I got to make a solution that was modified from 1L to 100ml. I got to calibrate the scales and the pH meter. I tested the pH of my solution and topped it off with acid so that its pH would read 8.0. The only things that went off course today would be the fact one fish knew its fate of being homogenized and decide to take the glorious path of suicide. The other problem was that the solution buffer for the fish froze but i got to eat lunch so i'm rather happy with that. After homogenizing the fish I spun the 6 tubes and separated the pellet from the supernate and stored both acordingly in the -80 centigrade freezer.

Vacations!

Wonderful things vacations are.... Everyone seems to on one but me. So I sit and wait feeding and cleaning zebra fish routinely. in all I hope next week I'll get to grind up the fish and do a few assays.

The fish are alive!

I should use a script huh?

Not much to report

Well I've been a way from here for awhile, but I really don't have much to say other then one of our chemicals Bisphenol A arrived and I got to read the emergency over view and first aid measures. As for filler I'll say this Watson and Crick are called thieves by most people that learned about their DNA helix discovery all they did was just put the pieces that everyone came up with together. People that say they are thieves are merely jealous of the fact they haven't found a quick easy way to become a famous Nobel Prize winner,and are just to smug to admit that.

Weekend jitters

well after discovering the zebra fish need a day night cycle I bought a timed light switch and weekend feeders. So this weekends impact on Monday will be either more dead fish which will reduce us for six down to two if past loses repeat themselves, or everyone is alive and when I clean the tanks on Monday I won't be cleaning out half eaten fish bodies.